Journal: NeuroImage
Article Title: High-sensitivity detection of optogenetically-induced neural activity with functional ultrasound imaging
doi: 10.1016/j.neuroimage.2021.118434
Figure Lengend Snippet: (A) Computer-aided design schematic of the cranial window and optic fiber installation. (B) Image of a chronic cranial window installed on a mouse. (C) Coronal (left) and sagittal (right) view of a structural magnetic resonance image (MRI) with a cranial window and optic fiber implanted in the right primary motor cortex (M1). (D) Representative histological image of endogenous ChR2-YFP expression in layer V of M1. (E) Power Doppler image depicting the coronal field of view at bregma +0 mm. (F) Experimental timeline for fUSI, fMRI, and electrophysiological recordings in response to optogenetic stimulations. (G) Average fMRI (pre-cranial window; top, purple) and fUSI (bottom, green) time series from the bilateral primary motor cortex (M1) and striatum during right M1 stimulation at 10 Hz. Data are presented as mean (color) ± SEM (shaded region). Increasing darkness of the lines represents increasing stimulation intensities. The blue vertical bars indicate stimulation periods.
Article Snippet: Functional magnetic resonance imaging (fMRI) data was acquired with a Bruker BioSpec 70/30 USR small animal MR system (Bruker BioSpin MRI, Ettlingen, Germany) operating at 300MHz (7T).
Techniques: Expressing